In addition, growth of each organism may be semiquantitatively scored on the basis of growth in each of the streaked areas. Consequently, one or more of these areas should exhibit isolated colonies of the organisms contained in the specimen. Because the streaking procedure is, in effect, a “dilution” technique,diminishing numbers of microorganisms are deposited on the streaked areas. Result Interpretation on Tryptic Soy AgarĪfter incubation, most plates will show an area of confluent growth. For plated media, the medium side should be uppermost to prevent excessive accumulation of moisture on the agar surface. Tryptic Soy Agar formulations should be stored away from direct light at 4☌ to 8☌. Use the prepared media plates to inoculate the specimen to be cultured and then place in the incubator at optimum temperature.Allow the content to cool down to 40-45 ☌ and pour in the empty media plates under the strict aseptic atmosphere (preferably in Laminar Air Flow) and allowed it to cool at room temperature.Autoclave the content at 121 ☌ and 15 psi pressure for 15 minutes.
Mix well the content and apply the Non-absorbent cotton plug to the flask.
If required, adjust the pH by adding either 1N HCl (acid) or 1N NaOH (base) as per the case.
Weigh the quantity of Pancreatic digest of casein, Papaic digest of soybean meal, dextrose, Dipotassium Hydrogen Phosphate, sodium chloride, and Agar-Agar using the weighing scale for 1000 ml of Trypticase Soy Agar medium.Sterile Conical Flask / Erlenmeyer Flask.For example, blood agar plates (BAP) are made by enriching TSA plates with defibrinated sheep blood, and chocolate agar is made through additional cooking of BAP. TSA is frequently the base medium of other agar plate types. As with any media, minor changes may be made to suit specific circumstances. The medium may be supplemented with blood to facilitate the growth of more fastidious bacteria or antimicrobial agents to permit the selection of various microbial groups from pure microbiota. Agar extracted from any number of organisms is used as a gelling agent. TSA contains enzymatic digests of casein and soybean meal, which provide amino acids and other nitrogenous substances, making it a nutritious medium for a variety of organisms. Glucose is the energy source. Sodium chloride maintains the osmotic equilibrium, while dipotassium phosphate acts as buffer to maintain pH. those bacteria which can grow easily on any medium and have no special requirements, are the Soybean and Casein Digest supported by various components. those bacteria that have some specific requirement for the growth as well as the Non-Fastidious bacteria i.e. The Tryptone Soy Agar (TSA) contains the magical ingredients that supports almost all types of Fastidious i.e.